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expand all sections collapse all sections  Oryza sativa QTL "CQF5" (soluble protein content)
QTL Accession ID CQF5
Species Oryza sativa (Rice) [ GR_tax:013681 ]
Trait Symbol SPROTCN
Trait Name soluble protein content
Published Symbol  
Trait Synonym(s) Soluble/Total protein
Trait Category Biochemical
Chromosome 3
Comments The soluble protein concentration in the supernatant was determined by the method of Bradford with bovine serum albumin as a standard. The protein extract was prepared from leaves by grinding them in liquid nitrogen to a fine powder and suspended in a buffer containing 100 mM NaH2PO4 (pH 7.0), 1 mM phenylmethylsulfonyl fluoride, 1% (w/v) insoluble polyvinyl polypyrrolidone, and 1% (v/v) beta-mercaptoethanol, and then centrifuged at 9,800 g for 5 min. The analysis was done on contents derived from leaf tissue collected 5 days after heading. The means of ten replications per line were used in the data analysis. Phenotypic effect contributed by Nipponbare allele. Chromosomal locations of putative QTLs were determined by single-factor analysis (one-way ANOVA) with the general linear model procedure of QGENE. A probability level of 0.01 was used as the threshold for detecting significant differences in the mean values of the two genotypic classes, homozygous for Nipponbare and Kasalath alleles. In the back crossed inbred lines (BILs) there was a high correlation among the soluble protein content and Rubisco content at 5 days (r=0.554; P<0.01) after heading.
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