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expand all sections collapse all sections  Oryza sativa QTL "CQF14" (rubisco to nitrogen content ratio)
QTL Accession ID CQF14
Species Oryza sativa (Rice) [ GR_tax:013681 ]
Trait Symbol RUBISCONICNRO
Trait Name rubisco to nitrogen content ratio
Published Symbol  
Trait Synonym(s)
Trait Category Biochemical
Chromosome 12
Comments Trait assayed for Rubisco to total leaf nitrogen content. Total leaf nitrogen content was determined by CN-Corder (Yanaco Co. Japan) by using the leaf samples oven-dried at 80C for 3 days. For determining Rubisco content soluble proteins (2 µg) were subjected to SDS-PAGE using 12.5% (w/v) gels containing 0.1% (w/v) SDS, and the gels were stained with Coomassie brilliant blue. The intensity of the band corresponding to Rubisco was measured with an image analyzer (Umax Technologies, Calif.). The analysis was done on contents derived from leaf tissue collected 5 days after heading. Phenotypic effect contributed by Kasalath allele. The means of ten replications per line were used in the data analysis. Chromosomal locations of putative QTLs were determined by single-factor analysis (one-way ANOVA) with the general linear model procedure of QGENE. A probability level of 0.01 was used as the threshold for detecting significant differences in the mean values of the two genotypic classes, homozygous for Nipponbare and Kasalath alleles. In the back crossed inbred lines (BILs) there was a high correlation among the soluble protein content and Rubisco content at 5 days (r=0.554; P<0.01) after heading
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