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expand all sections collapse all sections  Oryza sativa QTL "CQE82" (rubisco to chlorophyll ratio)
QTL Accession ID CQE82
Species Oryza sativa (Rice) [ GR_tax:013681 ]
Trait Symbol RUBISCOCHLRO
Trait Name rubisco to chlorophyll ratio
Published Symbol  
Trait Synonym(s)
Trait Category Biochemical
Chromosome 12
Comments Assay is an indicator of balance between the activities of Calvin cycle enzymes and electron carriers in the light reactions. The ratio of Chl-a to Chl-b (Chl a:b) were analyzed at 5 days after heading. For determining Rubisco content soluble proteins (2 µg) were subjected to SDS-PAGE using 12.5% (w/v) gels containing 0.1% (w/v) SDS, and the gels were stained with Coomassie brilliant blue. The intensity of the band corresponding to Rubisco was measured with an image analyzer (Umax Technologies, Calif.). The means of ten replications per line were used in the data analysis. Phenotypic effect contributed by Nipponbare allele. Chromosomal locations of putative QTLs were determined by single-factor analysis (one-way ANOVA) with the general linear model procedure of QGENE. Two-way ANOVAs for epistatic interaction were also carried out with QGENE at Pd0.01 threshold. The tests were done only with marker loci linked to QTLs with a single-factor P of d0.01 to reduce the likelihood of committing a type-I error.
toggle section  Map Positions (2)
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