ID | 771643 | ||||||
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Name | BF202385 (GenBank Accession ) | ||||||
Synonyms (6) |
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GenBank GI | 11117127 | ||||||
GenBank Version | BF202385.1 | ||||||
Type | EST | ||||||
Species | Triticum aestivum (Wheat) [ GR_tax:014341 ] | ||||||
Germplasm | |||||||
Description | WHE1764_A07_A14ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1764_A07_A14, mRNA sequence. |
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Tags () | None |
Marker Created: | |
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Marker Updated: | |
Internal Marker Accession: | BF202385 |
Clone | WHE1764_A07_A14 |
Comment |
Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer. |
Date Created | |
Date Updated | |
Lab Host | E. coli SOLR |
Map | |
Note |
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). |
Origin | |
Ref Authors |
Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C. |
Ref Location | Unpublished (2000) |
Ref Medline | |
Ref Pubmed | |
Ref Title |
The structure and function of the expressed portion of the wheat genomes - Pre- anthesis spike cDNA library |
Ref Year | 2000 |
GACTGTCAGGGGGAGAGCGATCTTTTTCGACACTTTGCTTCGCTTTAGCACTTCATGGGATGATAGAAGCACCTTTTAGG
GCCATGGATGAGTTTGATGTATTCATGGACGCGGTGAGCTGTAAAATAAGCTTGGACACCCTTGTAGATTTTGCTGTTGC
ACAAGGCTCACAATGGATATTTATAACACCGCATGATATCAGCATCGTGAAGCCTGGAGATCACAAATGGAAAATCGCCT
GTCTGTTACTGTGCTCAGTAGTGTATATATCTCCTTAGCCTTAGCATCCACGTTTGACAGAACCTGTCGGGAACTGCTCG
CCTTGTTTTTAAGTTGTGCATTGCTTGTTTCCAAATGTGAAC
Map Type | Map Set | Name | Map | Position | Ext. Links | |||
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Physical | IWGSC Physical 3B | BNL6.32 | 3B | 614,897,449-614,897,449 bp | View Comparative Map | |||
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Map Type | Map Set | Name | Map | Position | Ext. Links | |||
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Sequence | Gramene Annotated Nipponbare Sequence 2009 | BNL6.32 | Chr. 9 | 1,654,839-1,655,055 bp | View Comparative Map | |||
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Species | Type | Name | Assoc. Type |
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Triticum aestivum | Breakpoint Interval | 3DS6-0.55-1.00 | Wheat-EST-BI |
Triticum aestivum | Breakpoint Interval | C-3BS1-0.33 | Wheat-EST-BI |
Triticum aestivum | Breakpoint Interval | C-1BL6-0.32 | Wheat-EST-BI |