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expand all sections collapse all sections  Oryza sativa QTL "CQF11"
ID 50837
Name CQF11 (QTL ACCESSION {description)
Synonyms (1) CQF11-RUBISCOCN
TO TERM {descriptionTO:0000319 (rubisco content)
Trait Namerubisco content
Trait SymbolRUBISCOCN
Type QTL
Species Oryza sativa (Oryza sativa) [ GR_tax:013681 ]
Germplasm
Description For determining Rubisco content soluble proteins (2 µg) were subjected to SDS-
PAGE using 12.5% (w/v) gels containing 0.1% (w/v) SDS, and the gels were stained
with Coomassie brilliant blue. The intensity of the band corresponding to
Rubisco was measured with an image analyzer (Umax Technologies, Calif.). The
analysis was done on contents derived from leaf tissue collected 5 days after
heading. Phenotypic effect contributed by Kasalath allele. The means of ten
replications per line were used in the data analysis. Chromosomal locations of
putative QTLs were determined by single-factor analysis (one-way ANOVA) with the
general linear model procedure of QGENE. A probability level of 0.01 was used as
the threshold for detecting significant differences in the mean values of the
two genotypic classes, homozygous for Nipponbare and Kasalath alleles. In the
back crossed inbred lines (BILs) there was a high correlation among the soluble
protein content and Rubisco content at 5 days (r=0.554; P<0.01) after heading.
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