ID | 50768 |
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Name | CQF8 (QTL ACCESSION ) |
Synonyms (1) | CQF8-SPROTCN |
TO TERM | TO:0000325 (soluble protein content) |
Trait Name | soluble protein content |
Trait Symbol | SPROTCN |
Type | QTL |
Species | Oryza sativa (Oryza sativa) [ GR_tax:013681 ] |
Germplasm | |
Description | The soluble protein concentration in the supernatant was determined by the method of Bradford with bovine serum albumin as a standard. The protein extract was prepared from leaves by grinding them in liquid nitrogen to a fine powder and suspended in a buffer containing 100 mM NaH2PO4 (pH 7.0), 1 mM phenylmethylsulfonyl fluoride, 1% (w/v) insoluble polyvinyl polypyrrolidone, and 1% (v/v) beta-mercaptoethanol, and then centrifuged at 9,800 g for 5 min. The analysis was done on contents derived from leaf tissue collected 25 days after heading. The means of ten replications per line were used in the data analysis. Phenotypic effect contributed by Nipponbare allele. Chromosomal locations of putative QTLs were determined by single-factor analysis (one-way ANOVA) with the general linear model procedure of QGENE. A probability level of 0.01 was used as the threshold for detecting significant differences in the mean values of the two genotypic classes, homozygous for Nipponbare and Kasalath alleles. In the back crossed inbred lines (BILs) there was a high correlation among the soluble protein content and Rubisco content at 25 days (r=0.248; P<0.05) after heading. |
Tags () | None |
Marker Created: | |
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Marker Updated: | |
Internal Marker Accession: | CQF8 |
Chromosome | 6 |
Map Type | Map Set | Name | Map | Position | Ext. Links | |||
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Sequence | Gramene Annotated Nipponbare Sequence 2009 | BNL6.32 | Chr. 6 | 13,898,277-24,919,236 bp | View Comparative Map | |||
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Map Type | Map Set | Name | Map | Position | Ext. Links | |||
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QTL | JRGP Nip/Kas F2 QTL 2000 | BNL6.32 | 6 | 69.6-91.9 cM | View Comparative Map | |||
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Term Type | Term Name | Term Accession | |
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Plant Structure | flag leaf | PO:0020103 | Search |
Plant Growth and Development Stage | whole plant fruit ripening complete stage | PO:0007038 | Search |
Growth Stage | 09-mature grain stage | GRO:0007045 | Search |
Environment | unknown environment | EO:0007403 | Search |
Plant Structure | seed | PO:0009010 | Search |
Trait | soluble protein content | TO:0000325 | Search |
Taxonomy | Oryza sativa | GR_tax:013681 | Search |