Aegilops speltoides EST "BG274266"

4396

Name

BG274266 (GenBank Accession {description

)

Synonyms (4)

UNL165BG274266-1	UNL165BG274266-2
UNL165BG274266-4	WHE2226_E07_I14ZS

GenBank GI {description

13066405

GenBank Version {description

BG274266.1

Type

EST

Species

Aegilops speltoides [ GR_tax:012370 ]

Germplasm

Description

WHE2226_E07_I14ZS Aegilops speltoides anther cDNA library Aegilops speltoides
cDNA clone WHE2226_E07_I14, mRNA sequence.

Tags ()

None

Source/Library

Details

Marker Created:
Marker Updated:
Internal Marker Accession:	BG274266
Clone	WHE2226_E07_I14
Comment	Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer.
Date Created
Date Updated
Lab Host	E. coli SOLR
Map
Note	Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Origin
Ref Authors	Akhunov,E., Anderson,O.D., Chao,S., Chin,A., Choi,D.W., Close,T.J., Fenton,R.D, Han,P.S., Hsia,C.C., Kang,Y., Kianian,P., Lazo,G.R., Miller,R., Otto,C., Rausch,C.J., Seaton,C.L., Simons,K., Tong,J.C. and Zhang,D.
Ref Location	Unpublished (2000)
Ref Medline
Ref Pubmed
Ref Title	The structure and function of the expressed portion of the wheat genomes - Anther cDNA library from Aegilops speltoides
Ref Year	2000

Database Cross-References (0)

Sequences (1)

EST Sequence (1)

>BG274266 Aegilops speltoides EST [477 bp].

 
          TCGGAAACTACTGTTCAGCAGCTGAAGTATCTTGGATGTCGGTTGGAAAGGAGCAACTTGAATATGCATCTGAAGCTCTA
AAAAAGTTCAGATTTCTTGTGGAGCAGCTATCAAAAGTTAACCCTAATTGTTTGAACTCTGATGAGCGGCTAGCCTTTTG
GATTAACTTGTATAATGCGCTGATAATGCATTCATACCTAGCATACGGAGTTCCCCGGAATGACATCAAGCTTTTCTCTC
TAATGCAAAAGGCCTGTTACACAGTTGGTGGGCAGTCGTTCAGTGCAGCTGAAATAGAGTATGTGATACTAAAGATGAAG
ACTCCAGTGCACCGGCCCCAACTTTCTTTGATGTTGGCTCTTCAGAAGTTCAAAATTTCTGAGGGGCACAAGAAGTATTC
GATCGATCAAGCTGAGCCCCTTCTGCTGTTTGGTCTCAGTTGTGGAATGTTCTCTTCACCAGCTGTAAGAATCTTCA