Triticum aestivum EST "BF474224"

4242

Name

BF474224 (GenBank Accession {description

)

Synonyms (5)

BF474224P	UNL073BF474224-1
UNL073BF474224-2	UNL073BF474224-3
WHE0841_E12_I23ZS

GenBank GI {description

11543406

GenBank Version {description

BF474224.1

Type

EST

Species

Triticum aestivum (Wheat) [ GR_tax:014341 ]

Germplasm

Description

WHE0841_E12_I23ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA
clone WHE0841_E12_I23, mRNA sequence.

Tags ()

None

Source/Library

Details

Marker Created:
Marker Updated:
Internal Marker Accession:	BF474224
Clone	WHE0841_E12_I23
Comment	Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer.
Date Created
Date Updated
Lab Host	E. coli SOLR
Map
Note	Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Origin
Ref Authors	Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C.
Ref Location	Unpublished (2000)
Ref Medline
Ref Pubmed
Ref Title	The structure and function of the expressed portion of the wheat genomes - Vernalized crown cDNA library
Ref Year	2000

Database Cross-References (0)

Sequences (1)

EST Sequence (1)

>BF474224 Triticum aestivum EST [505 bp].

 
          GTGGTGGTGATGATGAGGATGACGATGATTGTGTGGTTCTAGATGGTGACCCTGAGAAGGCAATTACCATCGGGGAGGAC
AAGGGGAGTGTGGGAGATAGCAGCTCCGATGAATTGCAGATAGTCGGGGAGAAAGGACCGGTAGCGTGCAGGGACTTCCC
TCATTCACGCCATCTATGTTCTAACTTGCCCTTCAGCGCTACTTCTCACGTGAAGCATTGTAGCATGTGTCACTGTTTTG
TATGCGATGCCCCAGCTCCATGTAATTATTGGGGTAAAGGTATCCAGCTTAATGATCATTGTCATGCTACGGATAAGGAA
TCAAAGTGGAAACAGCTGCGGCAAGCATTCAAGTCTAAAAGTCTTCCAGCATCTCGTCCAGAACAATGCCAGAATGTCAT
CTACCCAACAATGGCACCATCCATTAAGAAGGATATACAGTGTCAAGTCTCGTCTTTTGCAAACAGAAGTCCTCTTCTGC
ATGTTATGAGCCAAAACAAACAAAG