Triticum aestivum EST "BE498513"

3848

Name

BE498513 (GenBank Accession {description

)

Synonyms (3)

UNL075BE498513-1	UNL075BE498513-2
WHE0967_A01_A01ZS

GenBank GI {description

9697130

GenBank Version {description

BE498513.1

Type

EST

Species

Triticum aestivum (Wheat) [ GR_tax:014341 ]

Germplasm

Description

WHE0967_A01_A01ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA
clone WHE0967_A01_A01, mRNA sequence.

Tags ()

None

Source/Library

Details

Marker Created:
Marker Updated:
Internal Marker Accession:	BE498513
Clone	WHE0967_A01_A01
Comment	Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer.
Date Created
Date Updated
Lab Host	E. coli SOLR
Map
Note	Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Origin
Ref Authors	Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C.
Ref Location	Unpublished (2000)
Ref Medline
Ref Pubmed
Ref Title	The structure and function of the expressed portion of the wheat genomes - Pre- anthesis spike cDNA library
Ref Year	2000

Database Cross-References (0)

Sequences (1)

EST Sequence (1)

>BE498513 Triticum aestivum EST [576 bp].

 
          TGCGAGCGTGGTTGTGGTGGCGTTCCGTGGCACGGAGCCATTCAACATGCAGGACTGGTCGACGGACGTGAACCTGTCGT
GGCTGGGCATGGGCGCCATGGGCCACGTCCACGCCGGCTTCCTCAAGGCGCTGGGCCTCCAGGAGGAGGACGGCAAGGAC
GCCAACCGCGCCTTCCCCAAGGACGCCCCCAACGGCGCCGCCCCAGTCGGCAAGGCCATCGCCTACTACAAGCTCCGCGA
GGTGATCCGCGACCAGCTCAAGGCGCACCCGCAGGCGCGGCTCGTGATCACCGGCCACAGCCTCGGCGGCGCGCTCGCCG
CCGTCTTCCCGGCGCTTCTGGCGCTGCACGGGGAGTCCGAGATCCTGGGCAGGCTCGGCACCGTGCAGACCTACGGGCAG
CCGCGCGTGGGCGACGCCACCTTCGTCAGCTTCTTCCGCGCCGAGGTGGAGAAGGCGACGGCCTTCTACCGCGTGGTGTA
CCGGTACGACGTGGTGTCGCGGGTGCCGTTCGACGCGCCGCCCGTCGCGGAGTTCGCCCACGGCGGCTCCTGCGTCTACT
ACGACGGGTGGTACGA