Triticum aestivum EST "BF478880"

3806

Name

BF478880 (GenBank Accession {description

)

Synonyms (9)

BF478880P	UCD141BF478880-1
UCD141BF478880-10	UCD141BF478880-2
UCD141BF478880-4	UCD141BF478880-5
UCD141BF478880-7	UCD141BF478880-9
WHE2014_G08_M16ZS

GenBank GI {description

11549707

GenBank Version {description

BF478880.1

Type

EST

Species

Triticum aestivum (Wheat) [ GR_tax:014341 ]

Germplasm

Description

WHE2014_G08_M16ZS Chinese Spring wheat drought stressed leaf cDNA library
Triticum aestivum cDNA clone WHE2014_G08_M16, mRNA sequence.

Tags ()

None

Source/Library

Details

Marker Created:
Marker Updated:
Internal Marker Accession:	BF478880
Clone	WHE2014_G08_M16
Comment	Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer.
Date Created
Date Updated
Lab Host	E. coli SOLR
Map
Note	Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Origin
Ref Authors	Akhunov,E., Anderson,O.D., Chao,S., Chin,A., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Kianian,P., Lazo,G.R., Miller,R., Nguyen H.T., Otto,C., Rausch,C.J., Seaton,C.L., Simons,K., Tong,J.C. and Zhang,D.
Ref Location	Unpublished (2000)
Ref Medline
Ref Pubmed
Ref Title	The structure and function of the expressed portion of the wheat genomes - Chinese Spring drought stressed leaf cDNA library
Ref Year	2000

Database Cross-References (0)

Sequences (1)

EST Sequence (1)

>BF478880 Triticum aestivum EST [497 bp].

 
          GGTTCATGCACTCACAGCTGCGGACACATGCTGAATCAATTGCTTTCTTTGGTGGTGGGTGCAAGAGAAAAAGCTTGGTT
GAGGCTAAATTCATGAAATTGCTTAACCACTCAAAGGTTCTGTTGAGAAAGCAATGGCTTTATGGTATTGTTGATGATTT
TGTGACAAAGCAACTCCCACACAATGTGACATGGGGTCTGAGTTTGTTATATGCTGTGGAGCACAANGGAGACAGAGCTT
TGACGTCAACACAAGGAGAGCTGGGGGATGCTCTGCGGTTCTTGGCATCAGTGGTGTCACAAAGCTTCATAGCCTTCGGT
GACATTCTTGAGTTACATAAGAAATTCCTTGAACTTTCTGGTGGGATTAACCGGATCTTTGAACTCCAGGAGATTCTGCG
TGTAGCTCAAAAGGATACCCCAGTGCCTTCCAGTGCCATTACTGCAGCCTCAGACGAAATTATTGAATTCCATGAAGTGG
ATATTGTAACACCATCG