Triticum aestivum EST "BF201923"

3800

Name

BF201923 (GenBank Accession {description

)

Synonyms (3)

BF201923P	NDS120BF201923-4
WHE1759-1762_H14_H14ZS

GenBank GI {description

11116665

GenBank Version {description

BF201923.1

Type

EST

Species

Triticum aestivum (Wheat) [ GR_tax:014341 ]

Germplasm

Description

WHE1759-1762_H14_H14ZS Wheat pre-anthesis spike cDNA library Triticum aestivum
cDNA clone WHE1759-1762_H14_H14, mRNA sequence.

Tags ()

None

Source/Library

Details

Marker Created:
Marker Updated:
Internal Marker Accession:	BF201923
Clone	WHE1759-1762_H14_H14
Comment	Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer.
Date Created
Date Updated
Lab Host	E. coli SOLR
Map
Note	Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Origin
Ref Authors	Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C.
Ref Location	Unpublished (2000)
Ref Medline
Ref Pubmed
Ref Title	The structure and function of the expressed portion of the wheat genomes - Pre- anthesis spike cDNA library
Ref Year	2000

Database Cross-References (0)

Sequences (1)

EST Sequence (1)

>BF201923 Triticum aestivum EST [490 bp].

 
          TTGCTTTCCCTCGTCTTCCTCCTGGCTACCTCGTCGGCGCGCGCCGGCGGGTTCAAGCACCTCCACCTGTACATGCACGA
GACCTTCACGGGCCCAAACGCCACGTTGTTTTCGGTGGTGCCGCCCCTGCACCGGGGCGACAACGCAAGGTTCGGGATGG
TTGGAGTGCTCGACGACGAGCTGCGCGACGGCCCGGACCCGAGAAACTCGTCTCTGGTCGGCCGGTTCCAGTCCCTCTTC
GCCTTCGCGGGGCTAGTGACGCCGCCGGGCATGCAGACGGCGACCAGCCTCGTGTTCACGGCCGGGGAGCACGCCGGGAG
CACGCTGGTCATGGTGGGCAGCATCGTGAGCTCCGAGGGCCCCTACGAGACCGCCGTGGTGGGCGGCACCGGCGCGTTTA
GGATGGCGCGCGGGTACTGCGTTCTGACGGCCGTGTGGAGCCCGACGCCGGAGTCCACCGTGTACGAGGTAAACCTGTTG
TTGAAGCTGG