Zea mays EST "T14655"

27752

Name

T14655 (GenBank Accession {description

)

Synonyms (2)

5C03G12

uaz151(sar)

GenBank GI {description

440634

GenBank Version {description

T14655.1

MaizeGDB cDNA - EST

p-5C03G12

Type

EST

Species

Zea mays (Maize) [ GR_tax:014450 ]

Germplasm

Description

05c03g12-t7 membrane-free polysomes from endosperm Zea mays cDNA clone 05c03g12
5' end similar to GTP-binding protein, mRNA sequence.

Tags ()

None

Source/Library

Details

Marker Created:
Marker Updated:
Internal Marker Accession:	T14655
Clone	05c03g12
Comment	Contact: The Maize cDNA Project Helentjaris TG (primary contact) Dept. of Plant Sciences University of Arizona Dept. of Plant Sciences, University of Arizona, Tucson, AZ,85721 ph: 602-6218-746 fax: 602-621-7186 E-mail: helnjars@ccit.arizona.edu Chris Baysdorfer Department of Biological Sciences, School of Science California State University, Hayward Hayward, CA 94542 ph: 510-881-3459 fax: 510-727-2035 E-mail: cbaysdor@s1.csuhayward.edu Rob Ferl Interdisciplinary Center for Biotechnology Research DNA Sequencing Core University of Florida P.O. Box 100695 Gainesville, FL 32611-0695 ph: 904-392- 1928, ext. 301 fax: 904-392-4072 E-mail: robferl@nervm.nerdc.ufl.edu Seq primer: T7.
Date Created
Date Updated
Lab Host	DH10B
Map
Note	Vector: ZipLox; Site_1: SalI; Site_2: NotI; ds-cDNA was prepared from oligo-dT selected mRNA by priming with a NotI oligo- dT oligomer and then adding the second strand to RNase-nicked DNA:RNA hybrid with DNA PolI. SalI adaptors were added to the ends, the ds-cDNAs were then digested with NotI and size-selected. These were directionally-cloned into the ZipLox phage vector, excised as plasmids, and then individually analyzed.
Origin
Ref Authors	Shen,B., Carneiro,N., Torres-Jerez,I., Stevenson,R., McCreery,T., Helentjaris,T., Baysdorfer,C., Almira,E., Ferl,R., Habben,J. and Larkins,B.
Ref Location	Plant Mol Biol 26, 1085-1101 (19
Ref Medline
Ref Pubmed	7811968
Ref Title	Partial sequencing and mapping of clones from two maize cDNA libraries
Ref Year	1994

Database Cross-References (2)

PubMed	7811968
Gramene Literature	Shen-B, Carneiro-N, Torres-Jerez-I, Stevenson-B, McCreery-T, Helentjaris-T, Baysdorfer-C, Almira-E, Ferl-R-J, Habben-J-E, "Partial sequencing and mapping of clones from two maize cDNA libraries", Plant molecular biology, 1994, vol. 26, pp. 1085-1101

Sequences (1)

EST Sequence (1)

>T14655 Zea mays EST [301 bp].

 
          NCAGATCCACACACATCCGCATCCCTCTCTCCTCGGTTTCCTCTCCCACCACCAACCCCCAGATCGCAGCGATCTCCGCC
GCCGCCCTCTCTGGGATTCCGCGCGACAAAGTCAGGCGTAGAGGCTCCAGGAGGAGGAGGGAGGCGCAGAGGGCGGGTGG
GGGAGATGTTCCTCTGGGACTGGTTCTACGGGGTGCTGGCCTCCCTCGGCCTGTGGCAGAAGGAGGCCAAGTTCCTCTTC
CTTGGCCTCGACAACGTCGGTAAGACCACGTTGTTCCACATGCTCAAGGACGAGCGGTTGG