Reference "cDNA structure and expression patterns of a low-temperature-specific wheat gene tacr7"

Reference ID

9228

Title

cDNA structure and expression patterns of a low-temperature-specific wheat gene tacr7

Source

Plant molecular biology, 1997, vol. 34, pp. 643-650

Authors (3)

Gana-J-A	Sutton-F
Kenefick-D-G

Abstract

The low-temperature (2 degrees C)-specific wheat cDNA, pTACR7, represents a gene
designated tacr7 from hard red winter wheat (HRWW; Triticum aestivum L. cv.
Winoka). The term low-temperature-specific (LTS) is used because tacr7 is not
induced by ABA or stresses such as salt, dehydration, and heat. pTACR7 was
isolated by RT-PCR with mRNA from wheat crown tissue, the oligonucleotide
primers derived from the barley cognate pHVCR8 (GenBank accession number
L28091). Based on the deduced amino acid sequence, TACR7 is highly hydrophobic,
with a single transmembrane domain and an amino acid bias for leucine (19%).
Thus, the encoded protein TACR7 is unique among low-temperature-regulated wheat
proteins described in the literature. Analysis of steady-state levels of tacr7
transcripts (630 nt) showed accumulation in wheat seedlings, crown tissue, and
callus cultures after transfer from control (25 degrees C) to low temperature (2
degrees C). No detectable transcripts were observed by northern blot
hybridization with pTACR7 probe from seedling or callus treated with ABA, salt,
dehydration, or heat stress. tacr7 transcripts accumulated during 2 degrees C
exposure to a greater amount in a freeze-resistant HRWW (FR; SDmut 16029) than
in a freeze-susceptible HRWW (FS; SDmut 16169) crown tissue, with the largest
difference between genotypes being 30% +/- 3% at 3 weeks.