Reference "The mitochondrial S13 ribosomal protein gene is silent in wheat embryos and seedlings"

Reference ID	9195
Title	The mitochondrial S13 ribosomal protein gene is silent in wheat embryos and seedlings
Source	Nucleic acids research, 1987, vol. 15, pp. 10393-10404
Author (1)	Bonen-L
Abstract	The sequence of a wheat mitochondrial reading frame encoding a protein homologous to the E. coli S13 small subunit ribosomal protein has been determined. The gene is located immediately downstream of a 1.4 kb recombinationally- active repeat element that contains the ATPase subunit 6 gene. The coding regions of the two genes are separated by only 153 bp, the shortest distance yet observed between protein-coding genes in plant mitochondria. However, their transcript profiles differ markedly. The ATPase 6 gene displays a single, prominent mRNA of approximately 1.4 kb, whereas the S13 gene shows no stable transcript as judged by Northern blot analysis of wheat mitochondrial RNA isolated from different developmental stages. A short segment of the 26S rRNA gene is located downstream of the S13 gene and its presence illustrates the frequent DNA duplication/rearrangements found in wheat mitochondria.