Reference "Characterisation of three cDNA clones encoding different mRNAs for the precursor to the small subunit of wheat ribulosebisphosphate carboxylase"

Reference ID

9191

Title

Characterisation of three cDNA clones encoding different mRNAs for the precursor to the small subunit of wheat ribulosebisphosphate carboxylase

Source

Nucleic acids research, 1983, vol. 11, pp. 8719-8734

Authors (3)

Smith-S-M	Bedbrook-J
Speirs-J

Abstract

We have isolated and sequenced three cDNA clones for the nuclear-encoded
precursor to the small subunit of the chloroplast enzyme, ribulose-1,5-
bisphosphate carboxylase of wheat. The nucleotide sequences of these clones are
different, indicating that they are probably derived from three different mRNAs.
This finding is consistent with the proposal that this polypeptide is encoded by
a multigene family in wheat, in support of similar data reported by Broglie et
al. (Bio/Technology 1:55-61, 1983). We deduce that the mature small subunit
polypeptide is comprised of 128 amino acids and that its precursor contains an
N-terminal transit peptide sequence. The sequences of both the mature small
subunit and its transit peptide differ at several positions from those
determined by Broglie et al, (1983) from a different wheat cultivar. Different
wheat cultivars might therefore contain different small subunit polypeptides. A
comparison of nucleotide and amino acid sequences of the small subunit from
wheat, pea, soybean and spinach shows that these sequences are not highly
conserved, particularly between monocotyledon and dicotyledon species.