Reference "Conservation and variability of wheat alpha/beta-gliadin genes"

Reference ID

9106

Title

Conservation and variability of wheat alpha/beta-gliadin genes

Source

Nucleic acids research, 1985, vol. 13, pp. 3905-3916

Authors (5)

Sumner-Smith-M	Rafalski-J-A
Sugiyama-T	Stoll-M
Soll-D

Abstract

We have sequenced two genomic clones for wheat alpha/beta-gliadin storage
protein genes. Comparison with a known sequence reveals close homology between
the three and confirms the previously suspected evolutionary relatedness of
members of this gliadin family. The coding region can be divided into six
domains. Two unusual structures were found within this region: (i) The P-boxes
which are composed of 12 codons, six of which are for proline, that are tandemly
repeated four or five times; and (ii) Two polyglutamine stretches which consist
of 18-22 tandemly repeated glutamine codons in one case, and 7-28 in the second.
Analysis of the P-box structures revealed that certain mutations were probably
present in the hypothetical ancestral alpha/beta-gliadin gene prior to gene
multiplication. None of the genes have introns. All of the genes appear to
contain typical eukaryotic promoters and also possess the double polyadenylation
signal of plants.