Reference "Editing of the wheat coxIII transcript: evidence for twelve C to U and one U to C conversions and for sequence similarities around editing sites"

Reference ID

9086

Title

Editing of the wheat coxIII transcript: evidence for twelve C to U and one U to C conversions and for sequence similarities around editing sites

Source

Nucleic acids research, 1990, vol. 18, pp. 3771-3776

Authors (3)

Gualberto-J-M	Weil-J-H
Grienenberger-J-M

Abstract

The complete cDNA sequence corresponding to the wheat coxIII gene transcript
(coding for subunit 3 of cytochrome oxidase) has been determined by a method
involving cDNA synthesis using specific oligonucleotides as primers followed by
PCR amplification, cloning and sequencing of the amplification products. In 12
different clones, the same 13 nucleotide modifications have been found as
compared to the genomic mitochondrial DNA sequence. Among these modifications,
12 are C----U conversions which change codons identities, thereby increasing the
homology between the wheat COXIII protein and the corresponding protein of non-
plant organisms. The 13th modification is a silent U----C conversion which seems
to be an unfrequent editing eventin plant mitochondria. Homologies can be found
between sequences surrounding editing sites in the coxIII transcript and in
other wheat mitochondrial transcripts. The presence of such homology suggests
that these sequences could base-pair with a common RNA molecule which might be
involved in editing site recognition.