Reference "Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase"

Reference ID

8976

Title

Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase

Source

Plant molecular biology, 1992, vol. 20, pp. 663-671

Authors (3)

Albert-H-A	Martin-T
Sun-S-S

Abstract

A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated
from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca.
6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp
downstream of the putative poly(A) addition signal is reported. A low-level,
essentially constitutive pattern of expression, amino acid sequence similarities
to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements
conserved in the upstream region of maize and sorghum C-4-specific PEPC genes
indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif
proposed to act as a phosphorylation site in light-mediated activation of
photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is
presented for the presence of this site in other housekeeping PEPC proteins.