Reference "Cloning, mapping and expression analysis of barley MADS-box genes"

Reference ID

8914

Title

Cloning, mapping and expression analysis of barley MADS-box genes

Source

Plant molecular biology, 2000, vol. 42, pp. 899-913

Authors (7)

Schmitz-J	Franzen-R
Ngyuen-T-H	Garcia-Maroto-F
Pozzi-C	Salamini-F
Rohde-W

Abstract

Six MADS-box cDNA clones were isolated by heterologous screening from a barley
inflorescence cDNA library. Based on sequence comparison to known MADS-box
genes, the barley MADS-box (BM) genes were grouped into three distinct
phylogenetic subclasses of the MADS-box gene family. The three MADS-box genes
BM3, BM5 and BM8 share similarities with genes of the SQUAMOSA (SQUA) subgroup,
while BM7 and BM9 belong to the AGAMOUS-LIKE 2 (AGL2) subgroup. BM1 resembles
MADS-box genes described as solitary sequences or orphan genes. Expression
analysis of the barley MADS-box genes revealed expression patterns that are not
characteristic of the barley MADS-box genes of the SQUA subgroup. while
expression of BM7 and BM9 was largely as expected for the AGL2 subgroup. BM1 is
mainly expressed in vegetative tissues and its primary transcript undergoes
alternative splicing such that the corresponding mRNAs differ by two codons. The
genes BM1, BM3 and BM8 were mapped by analysis of single-nucleotide
polymorphisms onto barley chromosomes 4, 2 and 7, respectively.