Reference "Control of transient expression of chimaeric genes by gibberellic acid and abscisic acid in protoplasts prepared from mature barley aleurone layers"

Reference ID

8900

Title

Control of transient expression of chimaeric genes by gibberellic acid and abscisic acid in protoplasts prepared from mature barley aleurone layers

Source

Plant molecular biology, 1991, vol. 16, pp. 713-724

Authors (2)

Jacobsen-J-V

Close-T-J

Abstract

Gibberellic acid (GA3) and abscisic acid (ABA) control the transcription of alpha-
amylase genes in barley aleurone cells. This control is likely to be exerted
through cis-acting hormone-responsive elements in the promoter region of the
gene. In order to further define these elements, we have developed procedures
for obtaining transient expression of chimaeric genes in protoplasts prepared
from mature barley aleurone layers. Constructs with heterologous constitutive
promoters and with heterologous and homologous GA3- and ABA-regulated promoters
were expressed specifically by these cells. This system would appear to offer
great potential in gene regulation studies especially for hormonally regulated
homologous genes. Functional analysis of a barley alpha-amylase gene has been
performed using this system. A 2050 bp fragment from a high-pI alpha-amylase
gene was fused to a reporter gene (GUS) and control of its expression was
examined. Deletion analysis of this promoter fragment showed that major GA- and
ABA-responsive elements occurred between 174 and 41 bp upstream from the
transcription initiation site.