Reference "The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases"

Reference ID

8877

Title

The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases

Source

Proceedings of the National Academy of Sciences of the United States of America, 2002, vol. 99, pp. 9328-9333

Authors (9)

Brueggeman-R	Rostoks-N
Kudrna-D	Kilian-A
Han-F	Chen-J
Druka-A	Steffenson-B
Kleinhofs-A

Abstract

Stem rust caused by Puccinia graminis f. sp. tritici was among the most
devastating diseases of barley in the northern Great Plains of the U.S. and
Canada before the deployment of the stem rust-resistance gene Rpg1 in 1942.
Since then, Rpg1 has provided durable protection against stem rust losses in
widely grown barley cultivars (cvs.). Extensive efforts to clone Rpg1 by synteny
with rice provided excellent flanking markers but failed to yield the gene
because it does not seem to exist in rice. Here we report the map-based cloning
and characterization of Rpg1. A high-resolution genetic map constructed with
8,518 gametes and a 330-kb bacterial artificial chromosome contig physical map
positioned the gene between two crossovers approximately 0.21 centimorgan and
110 kb apart. The region including Rpg1 was searched for potential candidate
genes by sequencing low-copy probes. Two receptor kinase-like genes were
identified. The candidate gene alleles were sequenced from resistant and
susceptible cvs. Only one of the candidate genes showed a pattern of apparently
functional gene structure in the resistant cvs. and defective gene structure in
the susceptible cvs. identifying it as the Rpg1 gene. Rpg1 encodes a receptor
kinase-like protein with two tandem protein kinase domains, a novel structure
for a plant disease-resistance gene. Thus, it may represent a new class of plant
resistance genes.