Reference "Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli"

Reference ID

8717

Title

Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli

Source

Molecular & General Genetics, 1989, vol. 217, pp. 355-361

Authors (7)

Schulz-R	Steinmuller-K
Klaas-M	Forreiter-C
Rasmussen-S	Hiller-C
Apel-K

Abstract

The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley
has been deduced from the nucleotide sequence of a cloned full-length cDNA. This
cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings
is drastically reduced upon illumination. The predicted amino acid sequence (388
residues in length) includes a transit peptide of 74 amino acids whose end point
has been delimited by sequencing the N-terminus of the mature protein.
Expression of the cDNA in Escherichia coli leads to the synthesis of an
enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase.
Activity of this protein in bacterial lysates is completely dependent on the
presence of NADPH and protochlorophyllide and requires light.