Reference "Barley lipid transfer protein, LTP1, contains a new type of lipid-like post-translational modification"

Reference ID

8707

Title

Barley lipid transfer protein, LTP1, contains a new type of lipid-like post-translational modification

Source

The Journal of biological chemistry, 2001, vol. 276, pp. 33547-33553

Authors (5)

Lindorff-Larsen-K	Lerche-M-H
Poulsen-F-M	Roepstorff-P
Winther-J-R

Abstract

In plants a group of proteins termed nonspecific lipid transfer proteins are
found. These proteins bind and catalyze transfer of lipids in vitro, but their
in vivo function is unknown. They have been suggested to be involved in
different aspects of plant physiology and cell biology, including the formation
of cutin and involvement in stress and pathogen responses, but there is yet no
direct demonstration of an in vivo function. We have found and characterized a
novel post-translational modification of the barley nonspecific lipid transfer
protein, LTP1. The protein-modification bond is of a new type in which an
aspartic acid in LTP1 is bound to the modification through what most likely is
an ester bond. The chemical structure of the modification has been characterized
by means of two-dimensional homo- and heteronuclear nuclear magnetic resonance
spectroscopy as well as mass spectrometry and is found to be lipid-like in
nature. The modification does not resemble any standard lipid post-translational
modification but is similar to a compound with known antimicrobial activity.