Reference "Characterization and sequence of a novel nitrate reductase from barley"

Reference ID

8701

Title

Characterization and sequence of a novel nitrate reductase from barley

Source

Molecular & General Genetics, 1991, vol. 228, pp. 329-334

Authors (6)

Miyazaki-J	Juricek-M
Angelis-K	Schnorr-K-M
Kleinhofs-A	Warner-R-L

Abstract

Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific
nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have
been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of
nitrate reductase was isolated from barley by homology to a partial-length NADH
nitrate reductase cDNA and the sequence determined. The open reading frame
encodes a polypeptide of 891 amino acids and its interrupted by two small
introns. The deduced amino acid sequence has 70% identity to the barley NADH-
specific nitrate reductase. The non-coding regions of the pMJ4.1 gene have low
homology (ca. 40%) to the corresponding regions of the NADH nitrate reductase
gene. Expression of the pMJ4.1 nitrate reductase gene is induced by nitrate in
root tissues which corresponds to the induction of NAD(P)H nitrate reductase
activity. The pMJ4.1 nitrate reductase gene is sufficiently different from all
previously reported higher plant nitrate reductase genes to suggest that it
encodes the barley NAD(P)H-bispecific nitrate reductase.