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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "DNA binding sites recognised in vitro by a knotted class 1 homeodomain protein encoded by the hooded gene, k, in barley (Hordeum vulgare)"
Reference ID 8679
Title DNA binding sites recognised in vitro by a knotted class 1 homeodomain protein encoded by the hooded gene, k, in barley (Hordeum vulgare)
Source FEBS letters, 1997, vol. 408, pp. 25-29
Authors (3)
Abstract The homeodomain of the knotted classes of transcription factors from plants
differs from the well characterized Antp/En type homeodomains from Drosophila at
key amino acid residues contributing to the DNA binding. A cDNA, Hvh21, derived
from the hooded gene and encoding a full length homolog of knotted1 from maize
was isolated from barley seedlings and expressed as a maltose binding protein
fusion in E. coli. The purified HvH21-fusion protein selected DNA fragments with
1-3 copies of the sequence TGAC. Gel shift experiments showed that the TGAC
element was required for binding and the results further indicate that the HvH21-
fusion protein binds DNA as a monomer.
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