grain_icon  Literature Home |  Rice Genetics Newsletters |  Tutorial |  FAQ
Find:
E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Separation of acidic barley endosperm proteins by two-dimensional electrophoresis"
Reference ID 8663
Title Separation of acidic barley endosperm proteins by two-dimensional electrophoresis
Source Electrophoresis, 1993, vol. 14, pp. 1060-1066
Author (1) Flengsrud-R
Abstract Proteins in barley aleurone and starchy endosperm from developing seeds were
separated by two-dimensional (2-D) electrophoresis according to O'Farrell's
method. The effect of different extraction methods on the separation was
compared. The two best extraction methods were compared by amino acid analysis
of extracts and residues. Some tissue-specific proteins in both tissues are
described. Different methods for transfer of separated proteins onto membranes
were compared. Amino terminal and internal sequences were determined after
transfer of separated proteins or their proteolytic products onto membranes.
Amino acid sequence data allowed the identification of 12 different proteins,
while 6 remain unidentified and 8 appeared to have a blocked amino terminal.
toggle section  Database Cross-References (1)
toggle section  Proteins (9)
box  Markers (0)
box  QTL (0)
box  Genes (0)
box  Ontologies (0)
box  Map Sets (0)
box  Diversity Experiments (0)

Please note:
To request reprints, please contact the authors or the source/journal website. Due to copyright issues Gramene does not distribute reprints.