Reference "Cloning of cDNA, expression, and chromosomal location of genes encoding the three types of subunits of the barley tetrameric inhibitor of insect alpha-amylase"

Reference ID

8660

Title

Cloning of cDNA, expression, and chromosomal location of genes encoding the three types of subunits of the barley tetrameric inhibitor of insect alpha-amylase

Source

Plant molecular biology, 1993, vol. 23, pp. 535-542

Authors (3)

Medina-J	Hueros-G
Carbonero-P

Abstract

Three cDNA clones from barley developing endosperm, corresponding to proteins
BTAI-CMa, BTAI-CMb and BTAI-CMd, which are the three types of subunits of the
tetrameric inhibitor of insect alpha-amylases, have been identified and
sequenced. The deduced amino acid sequence of BTAI-CMb corresponds to the
CM16/CM17 type of subunit in wheat (92/90% identical residues) and has one
putative N-glycosylation site (NLT) and a possible kinase-C phosphorylation site
(SCR). The BTAI-CMa sequence differs at four amino acid residues from a
previously reported one from cv. Bomi and the sequence deduced for BTAI-CMd
completes (11 N-terminal residues) and confirms previously available data. The
gene for BTAI-CMa (Iat1) is located in the beta arm of barley chromosome 7H
(syn.1), while genes for both BTAI-CMb (Iat2) and BTAI-CMd (Iat3) are in the
long arm of chromosome 4H. The three genes are expressed in endosperm and their
mRNAs are not detected in the other tissues tested, except Iat1, which seems to
be expressed at a low level in coleoptile and roots, where it is switched off by
50 microM methyl jasmonate.