Reference "Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by iron deficiency in barley roots"

Reference ID

8634

Title

Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by iron deficiency in barley roots

Source

Plant physiology, 1998, vol. 116, pp. 725-732

Authors (6)

Suzuki-K	Itai-R
Nakanishi-H	Nishizawa-N-K
Yoshimura-E	Mori-S

Abstract

To identify the proteins induced by Fe deficiency, we have compared the proteins
of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-
dimensional polyacrylamide gel electrophoresis. Peptide sequence analysis of
induced proteins revealed that formate dehydrogenase (FDH), adenine
phosphoribosyltransferase, and the lds3 gene product (for Fe deficiency-
specific) increased in Fe-deficient roots. FDH enzyme activity was detected in
Fe-deficient roots but not in Fe-sufficient roots. A cDNA encoding FDH (Fdh) was
cloned and sequenced. Fdh expression was induced by Fe deficiency. Fdh was also
expressed under anaerobic stress and its expression was more rapid than that
induced by Fe deficiency. Thus, the expression of Fdh observed in Fe-deficient
barley roots appeared to be a secondary effect caused by oxygen deficiency in
Fe-deficient plants.