Reference "Isolation and sequence analysis of a barley alpha-amylase cDNA clone"

Reference ID

8586

Title

Isolation and sequence analysis of a barley alpha-amylase cDNA clone

Source

The Journal of biological chemistry, 1983, vol. 258, pp. 8169-8174

Authors (2)

Rogers-J-C

Milliman-C

Abstract

We have isolated a cDNA clone derived from poly(A+) RNA from barley aleurone
cells stimulated with gibberellic acid. This cDNA clone contains one open
reading frame coding for 438 amino acids. The cloned DNA hybridizes to a
poly(A+) RNA species 1550 bases in size, the same size as the most abundant
poly(A+) RNA molecules in stimulated cells. RNA complementary to this clone can
be translated to make immunoprecipitable alpha-amylase in the wheat germ system
and increases about 5-fold in quantity after gibberellic acid stimulation of
aleurone cells. In contrast, hybridization experiments using a total cDNA probe
demonstrate that the most abundant mRNA population, identical in size with our
cloned sequence and presumably that for alpha-amylase, increases at least 17-
fold after gibberellic acid stimulation. We therefore infer that there must be
at least two populations of alpha-amylase mRNA molecules derived from separate
structural genes differently influenced by gibberellic acid in aleurone cells.