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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "Mass spectrometric analysis reveals a cysteine bridge between residues 2 and 61 of the auxin-binding protein 1 from Zea mays L"
Reference ID 8400
Title Mass spectrometric analysis reveals a cysteine bridge between residues 2 and 61 of the auxin-binding protein 1 from Zea mays L
Source FEBS letters, 2001, vol. 509, pp. 446-450
Authors (5)
Abstract The major auxin-binding protein (ZmERabp1) from maize (Zea mays L.) has been
structurally characterized. We determined the position of a disulfide bridge in
ZmERabp1 by mass-spectrometric analysis. We show that Cys2 and Cys61 are
covalently linked and that residue Cys155 bears the free sulfhydryl group. By
making use of electrospray mass spectrometry, the molecular mass of ZmERabp1 was
determined to be 20,243 Da comprising a sugar moiety of 1865 Da, corresponding
to a high mannose-type glycan structure. Due to the high homology among all
characterized ABPs, the information on the disulfide bonds will be important for
functional analysis of recombinantly expressed ABP1.
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