Reference "Phosphorus deficiency-induced root elongation and its QTL in rice ( Oryza sativa L.)"

Reference ID

8342

Title

Phosphorus deficiency-induced root elongation and its QTL in rice ( Oryza sativa L.)

Source

Theoretical and applied genetics, 2004, vol. 109, pp. 1361-1368

Authors (4)

Shimizu-A	Yanagihara-S
Kawasaki-S	Ikehashi-H

Abstract

A significant level of root elongation was induced in rice ( Oryza sativa) grown
under phosphorus-deficient conditions. The root elongation clearly varied among
a total of 62 varieties screened under two different phosphorus levels. Two
contrasting varieties, 'Gimbozu', with a low elongating response and 'Kasalath',
with a high elongating response, were chosen and crossed to produce a hybrid
population for QTL analyses. QTLs for the phosphorus deficiency-induced root
elongation were detected by two linkage maps, i.show $132#e., one with 82 F(3)
families constructed by 97 simple sequence repeat (SSR) and sequence-tag site
markers and another with 97 F(8) lines by 790 amplified fragment length
polymorphism and SSR markers. A single QTL for the elongation response was
detected on chromosome 6, with a LOD score of 4.5 in both maps and explained
about 20% of total phenotypic variance. In addition, this QTL itself, or a
region tightly linked with it, partly explained an ability to reduce
accumulation of excess iron in the shoots. The identified QTL will be useful to
improve rice varieties against a complex nutritional disorder caused by
phosphorus deficiency and iron toxicity.