Reference "Fine genetic mapping of a gene required for Rice yellow mottle virus cell-to-cell movement"

Reference ID

8266

Title

Fine genetic mapping of a gene required for Rice yellow mottle virus cell-to-cell movement

Source

Theoretical and applied genetics, 2003, vol. 107, pp. 371-378

Authors (8)

Albar-L	Ndjiondjop-M-N
Esshak-Z	Berger-A
Pinel-A	Jones-M
Fargette-D	Ghesquiere-A

Abstract

The very high resistance to Rice yellow mottle virus observed in the two rice
varieties Gigante ( Oryza sativa) and Tog 5681 ( O. glaberrima) is monogenic and
recessive. Bulked segregant analysis was carried out to identify AFLP markers
linked to the resistance gene. Mapping of PCR-specific markers, CAPS and
microsatellite markers on 429 individuals of an IR64 x Gigante F(2) population
pinpointed this resistance gene on the long arm of chromosome 4 in a 3.7-cM
interval spanned by PCR markers. These markers also flanked the resistance gene
of the O. glaberrima accession Tog 5681 and confirmed previous allelism tests.
The rarity of this recessive natural resistance was in line with a resistance
mechanism model based on point mutations of a host component required for cell-to-
cell movement of the virus. Preliminary data on the genetic divergence between
the two cultivated rice species in the vicinity of the resistance locus
suggested that two different resistance alleles are present in Gigante and Tog
5681. A large set of recombinants is now available to envisage physical mapping
and cloning of the gene.