Reference "The genetic basis of stay-green in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross"

Reference ID

8186

Title

The genetic basis of stay-green in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross

Source

Theoretical and applied genetics, 2004, vol. 108, pp. 688-698

Authors (5)

Jiang-G-H	He-Y-Q
Xu-C-G	Li-X-H
Zhang-Q

Abstract

Delayed leaf-senescence, or stay-green, has been regarded as a desired
characteristic for the production of a number of crops including rice. In this
study, we analyzed the genetic basis of stay-green using a population of 190
doubled haploid lines from the cross between an indica parent Zhenshan 97 and a
stay-green japonica parent Wuyujing 2. The population was tested in replicated
field trials in 2 consecutive years, and six traits were defined to evaluate the
stay-green characteristics. A genetic linkage map with 179 SSR (simple sequence
repeat) marker loci was constructed. The software QTLMapper, based on a mixed
linear model approach, was applied to detect QTLs, epistatic effects and their
environmental interactions for these traits. A total of 46 main-effect QTLs was
detected for the six traits that can be localized to 25 chromosomal regions. The
individual effects of all the QTLs were small. Fifty digenic interactions were
resolved that involved 66 loci distributed on all 12 chromosomes. Environmental
interactions were detected for 18 of the main-effect QTLs and 14 of the
epistatic interactions. Collectively, the epistatic effects and QTL by year
interactions accounted for large proportions of the phenotypic variations. The
results also showed that most of the stay-green traits were negatively
correlated with yield and its component traits. The implications of the results
in crop improvement were discussed.