Reference "The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice"

Reference ID

8000

Title

The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice

Source

The Plant cell, 2003, vol. 15, pp. 1728-1739

Authors (7)

Nonomura-K	Miyoshi-K
Eiguchi-M	Suzuki-T
Miyao-A	Hirochika-H
Kurata-N

Abstract

The function of the novel gene MSP1 (MULTIPLE SPOROCYTE), which controls early
sporogenic development, was elucidated by characterizing a retrotransposon-
tagged mutation of rice. The MSP1 gene encoded a Leu-rich repeat receptor-like
protein kinase. The msp1 mutation gave rise to an excessive number of both male
and female sporocytes. In addition, the formation of anther wall layers was
disordered and the tapetum layer was lost completely. Although the mutation
never affected homologous chromosome pairing and chiasma maintenance, the
development of pollen mother cells was arrested at various stages of meiotic
prophase I, which resulted in complete male sterility. Meanwhile, plural
megaspore mother cells in a mutant ovule generated several megaspores,
underwent gametogenesis, and produced germinable seeds when fertilized with wild-
type pollen despite disorganized female gametophytes. In situ expression of
MSP1 was detected in surrounding cells of male and female sporocytes and some
flower tissues, but never in the sporocytes themselves. These results suggest
that the MSP1 product plays crucial roles in restricting the number of cells
entering into male and female sporogenesis and in initiating anther wall
formation in rice.