Reference "Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis"

Reference ID

7682

Title

Hypoxically inducible barley alanine aminotransferase: cDNA cloning and expression analysis

Source

Plant molecular biology, 1994, vol. 24, pp. 417-427

Authors (2)

Muench-D-G

Good-A-G

Abstract

A 1.75 kb cDNA containing the entire coding sequence of the hypoxically
inducible alanine aminotransferase (AlaAT) from barley roots was isolated and
sequenced. This clone has an open reading frame of 1446 bp, and a deduced amino
acid sequence of 482 residues, giving an estimated protein molecular mass of
52,885 Da. RNA blot analysis of barley root tissue showed a 4-fold increase of a
single AlaAT-2 mRNA band after 12-24 hours of hypoxic stress, followed by a
decrease in message levels after 48 h of hypoxic conditions. AlaAT-2 protein
concentration increased in a similar pattern to AlaAT activity in root tissue,
to almost 6-fold the aerobic level after 96 h of hypoxic stress. AlaAT-2
activity increased more than 2-fold in roots of Panicum miliaceum exposed to
hypoxia, and is the same isoform as the light inducible AlaAT in P. miliaceum
leaves. The unique expression patterns of AlaAT-2 in root and leaf tissue upon
exposure to different environmental stimuli is also discussed.