Reference "Analysis of distal flanking regions of maize 19-kDa zein genes"

Reference ID

7402

Title

Analysis of distal flanking regions of maize 19-kDa zein genes

Source

Gene, 1989, vol. 80, pp. 249-258

Authors (3)

Quayle-T-J	Brown-J-W
Feix-G

Abstract

Two genomic fragments from maize, each containing a 19-kDa zein gene with
extensive flanking regions, have been sequenced and examined by computer-aided
analysis and Southern blotting techniques. Sequence analysis of the distal
flanking sequences has revealed interesting sequence motifs, some not seen
before. In particular, four nearly identical, G + C-rich, 17 to 21-bp perfect
palindromes were found clustered in a 133-bp stretch lying 2 kb upstream from
the zein-coding region in the genomic clone pMS2. These palindromic sequences
exhibit other interesting features, including a precise spatial organization
with respect to each other, and their proximity to several other repeated motifs
in the same region. Southern blot analysis indicates that these palindromes, or
closely related sequences, are found frequently in the maize genome. Possible
secondary structures for the palindrome units are presented, which resemble
functionally important sequences found upstream from other eukaryotic genes.