Reference "Nucleotide sequence analysis of zein mRNAs from maize endosperm"

Reference ID

7399

Title

Nucleotide sequence analysis of zein mRNAs from maize endosperm

Source

The Journal of biological chemistry, 1985, vol. 260, pp. 16451-16459

Authors (3)

Marks-M-D	Lindell-J-S
Larkins-B-A

Abstract

A comparison of the DNA and protein sequences of a group of zein cDNA clones
reveals that they share extensive sequence homology and probably originated from
a common ancestral gene. A comparison of clones corresponding to Mr 22,000
polypeptides shows they are 92% homologous, while five clones corresponding to
the Mr 19,000 zeins vary in homology from 75 to 95%. The clones corresponding to
the Mr 22,000 proteins are 60-65% homologous to clones encoding the Mr 19,000
zein proteins. A clone corresponding to the Mr 15,000 zein has little homology
to either the Mr 22,000 or 19,000 zeins. Clones corresponding to both the Mr
22,000 and 19,000 zeins have two putative polyadenylation signals. S1 nuclease
mapping indicates that the first polyadenylation signal following the stop codon
is utilized by the Mr 22,000 sequences, while primarily the second
polyadenylation signal is utilized by the Mr 19,000 sequences.