Reference "Cloning and sequence analysis reveal structural variation among related zein genes in maize"

Reference ID

7398

Title

Cloning and sequence analysis reveal structural variation among related zein genes in maize

Source

Cell, 1982, vol. 29, pp. 1015-1026

Authors (5)

Pedersen-K	Devereux-J
Wilson-D-R	Sheldon-E
Larkins-B-A

Abstract

We have isolated a gene encoding one of the 19,000 dalton zein proteins from a
maize genomic library constructed in Charon 4A. This gene occurs on a 7.7 kb Eco
RI fragment, and based on Southern hybridization analysis, represents one of
several homologous sequences present in the maize genome. The nucleotide
sequence of the gene predicts a protein composed of 235 amino acids, including a
signal peptide of 21 amino acids. There are no intervening sequences in the
gene. By comparing the nucleotide sequence of this gene with that of a
homologous cDNA clone, we have identified a basis for microheterogeneity within
the gene family. The 5' nucleotide sequences of the genomic and cDNA clones are
identical, but they differ in the center of the protein, where repeated amino
acid sequences occur. A nucleotide sequence encoding a conserved peptide of 20
amino acids is repeated nine times in the center of both of these clones.