Reference "Primary structure of a genomic zein sequence of maize"

Reference ID

7396

Title

Primary structure of a genomic zein sequence of maize

Source

The EMBO journal, 1982, vol. 1, pp. 1337-1342

Authors (5)

Hu-N-T	Peifer-M-A
Heidecker-G	Messing-J
Rubenstein-I

Abstract

The nucleotide sequence of a genomic clone (termed Z4 ) of the zein multigene
family was compared to the nucleotide sequence of related cDNA clones of zein
mRNAs. A tandem duplication of a 96-bp sequence is found in the genomic clone
that is not present in the related cDNA clones. When the duplication is
disregarded, the nucleotide sequence homology between Z4 and its related cDNAs
was approximately 97%. The nucleotide sequence is also compared to other
isolated cDNAs. No introns in the coding region of the zein gene are detected.
The first nucleotide of a putative TATA box, TATAAATA , was located 88
nucleotides upstream of the first nucleotide of the first ATG codon which
initiated the open reading frame. The first nucleotide of a putative CCAAT box,
CAAAAT , appeared 45 nucleotides upstream of the first nucleotide of the zein
cDNA clones in the 3' non-coding region also appeared in the genomic sequence at
the same locations. The amino acid composition of the polypeptide specified by
the Z4 nucleotide sequence is similar to the known composition of zein proteins.