Reference "Isolation and characterization of a cDNA for mitochondrial manganese superoxide dismutase (SOD-3) of maize and its relation to other manganese superoxide dismutases"

Reference ID

7364

Title

Isolation and characterization of a cDNA for mitochondrial manganese superoxide dismutase (SOD-3) of maize and its relation to other manganese superoxide dismutases

Source

Biochimica et biophysica acta, 1988, vol. 951, pp. 61-70

Authors (2)

White-J-A

Scandalios-J-G

Abstract

We describe the isolation of a cDNA clone for the nuclear-encoded manganese
superoxide dismutase (SOD-3) of maize mitochondria. The cDNA, pSod3.1c, selects
by hybridization an RNA which produces the SOD-3 precursor upon in vitro
translation. The DNA sequence of pSod3.1c was determined from fragments
subcloned in M13. The amino-acid sequence deduced from the nucleotide sequence
displays significant homology with the amino-acid sequences of prokaryotic and
eukaryotic Mn-SODs, but displays greater homology with mammalian Mn-SOD than it
does with yeast or bacterial Mn-SOD. A 31 amino-acid transit peptide also is
encoded by the pSod3.1c clone. Analysis of poly(A)+ RNA indicates that Sod3 mRNA
is approx. 1250 nucleotides in length. The amount of Sod3 transcript in seedling
leaves is increased by light.