Reference "Cloning of cDNA for maize superoxide dismutase 2 (SOD2)"

Reference ID

7362

Title

Cloning of cDNA for maize superoxide dismutase 2 (SOD2)

Source

Proceedings of the National Academy of Sciences of the United States of America, 1987, vol. 84, pp. 179-183

Authors (3)

Cannon-R-E	White-J-A
Scandalios-J-G

Abstract

A cDNA clone encoding maize cytosolic superoxide dismutase 2 (SOD2) was isolated
from a cDNA library constructed in pUC9 plasmids from size-selected poly(A)+
RNA. The library was screened with mixed synthetic oligode-oxynucleotide probes.
The sequence of the probes was derived from the amino acid sequence from a
region of the protein near the NH2 terminus. One positive clone contained an
insertion of a 612-base-pair fragment. The amino acid sequence, deduced from the
nucleotide sequence of the cDNA, revealed that the clone contained the coding
region for all but the first of the 151 amino acids of the SOD2 protein.
Additional 5' and 3' flanking sequences, absent on the pUC9 Sod2 clone, were
obtained from a lambda gt11 clone isolated from a maize leaf library probed with
the pUC9 Sod2 insert. Hybrid-selection translation assays also demonstrate that
the cDNA clone contains Sod2 sequences.