Reference "Nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from maize"

Reference ID

7326

Title

Nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from maize

Source

Journal of biochemistry, 1987, vol. 102, pp. 673-676

Authors (5)

Matsuoka-M	Kano-Murakami-Y
Tanaka-Y	Ozeki-Y
Yamamoto-N

Abstract

We have cloned a full length cDNA for the small subunit of ribulose-1,5-
bisphosphate carboxylase from C4 monocot maize, determined the complete
nucleotide sequence of this cDNA and deduced its amino acid sequence. The cDNA
insert included 513 bp of the coding region, and 65 and 252 nucleotides of the
5' and 3' untranslated regions, respectively. The transit and mature peptides
have, respectively, 47 and 123 amino acids. Comparison with the small subunit
genes from other plants revealed that the maize small subunit is similar to the
wheat one, there being 73% homology between the transit peptides and 64% between
the mature proteins. This indicates that there is no noteworthy difference
between the C3 and C4 small subunit structures. Extreme codon bias was observed
for this gene, and similar codon preferences are observed for other proteins
highly expressed in maize leaf, light harvesting chlorophyll binding protein and
phosphoenolpyruvate carboxylase. The results indicate that preferential codon
usage for highly expressed genes occurs in maize leaf.