Reference "Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of glutathione S-transferase isoforms I, II and IV"

Reference ID

7216

Title

Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of glutathione S-transferase isoforms I, II and IV

Source

Plant molecular biology, 1994, vol. 26, pp. 1855-1866

Authors (5)

Jepson-I	Lay-V-J
Holt-D-C	Bright-S-W
Greenland-A-J

Abstract

Several GSTs have been characterised in maize. GST I is a homodimer of 29 kDa
subunits, GST II a hetrodimer of 27 kDa and 29 kDa subunits and GST IV a
homodimer of 27 kDa subunits. We report the isolation and characterization of a
herbicide-safener inducible cDNA clone, GST-27. Based on partial amino acid
sequence, GST-27 encodes the 27 kDa subunit present in both glutathione S-
transferase isoforms GST II and IV. Northern blotting was used to compare the
expression patterns of GST-27 with that of GST-29. Transcripts corresponding to
GST-27 were found to be constitutively expressed in RNA isolated from the root,
but no expression was detected in RNA isolated from aerial parts of the plant.
The application of herbicide safener caused a dramatic increase in the
expression of GST-27 in all aerial plant parts tested. GST-29 was found to be
constitutively expressed in RNA isolated from a number of maize tissues. The
basal level of GST-29 expression showed a minimal increase upon herbicide
safener treatment. Although a range of hormonal, environmental and physiological
stimuli failed to elevate GST-27 levels, some increase in GST-27 mRNA was
observed in the late stages of leaf senescence and after treatments resulting in
phytotoxic effects.