Reference "Cloning and expression of a cDNA encoding a maize glutathione-S-transferase in E. coli"

Reference ID

7215

Title

Cloning and expression of a cDNA encoding a maize glutathione-S-transferase in E. coli

Source

Nucleic acids research, 1986, vol. 14, pp. 7227-7235

Authors (6)

Moore-R-E	Davies-M-S
O'Connell-K-M	Harding-E-I
Wiegand-R-C	Tiemeier-D-C

Abstract

The isolation and characterization of a family of maize glutathione-S-
transferases (GST's) has been described previously. These enzymes are designated
GSTs I, II and III based on size, substrate specificity and responsiveness to
safeners. GST III has been shown to act on the herbicide alachlor as well as the
commonly used substrate 1-chloro-2,4-dinitrobenzene (CDNB). Clones were isolated
from a maize cDNA library in lambda gt10. Three clones contained the entire
coding region for GST III. The sequences of these clones were consistent with
the known amino terminal GST III protein sequence. Moreover, expression of one
of these clones in E. coli resulted in a GST activity as measured with both CDNB
and alachlor, proving that at least one of the clones encodes an active GST III
species. With the enzyme expressed in E. coli it will become possible to study
enzyme structure-function relationships ex planta. While a number of different
GST proteins are present in maize tissue the GST III gene is present in single
or low copy in the genome.