Reference "Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays"

Reference ID

7139

Title

Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays

Source

Proceedings of the National Academy of Sciences of the United States of America, 1991, vol. 88, pp. 3377-3381

Authors (3)

Colasanti-J	Tyers-M
Sundaresan-V

Abstract

We describe the isolation of cDNA clones encoding a p34cdc2 homologue from a
higher plant, Zea mays (maize). A full-length cDNA clone, cdc2ZmA, was isolated,
sequenced, and shown to complement a cdc28 mutation in Saccharomyces cerevisiae.
Comparison of the deduced amino acid sequence of the maize p34cdc2 protein with
other homologues showed that it was 64% identical to human p34cdc2 and 63%
identical to Schizosaccharomyces pombe and S. cerevisiae p34cdc2 proteins.
Studies of expression of the maize cdc2 gene(s) by Northern blot analysis
indicated a correlation between the abundance of cdc2 mRNA and the proliferative
state of the tissue. Southern blot analysis, as well as isolation of another
cDNA clone, cdc2ZmB, which is 96% identical to cdc2ZmA, indicates that maize has
multiple cdc2 genes.