Reference "cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods"

Reference ID

7083

Title

cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods

Source

The EMBO journal, 1989, vol. 8, pp. 2463-2467

Authors (8)

Tillmann-U	Viola-G
Kayser-B	Siemeister-G
Hesse-T	Palme-K
Lobler-M	Klambt-D

Abstract

An auxin-binding protein (ABP) cDNA clone was selected from a lambda gt11 cDNA
library from corn coleoptiles with highly purified IgGanti ABP. The sequence of
794 bp contains an open reading frame (ORF) of 603 bp, coding for a 22 kd
protein. There are indications of a signal peptide of 38 amino acids (von
Heijne, G. 1983, Eur. J. Biochem., 133, 17-21). A N-glycosylation site can be
deduced and a C-terminal KDEL amino acid sequence is detected. An EcoRI fragment
containing the beginning portion of the cDNA with about three quarters of the
ORF was used to select cDNA clones from an independently produced lambda gt11
cDNA library of corn coleoptiles. Northern blot analysis with in vitro
transcribed biotinylated RNA showed a single band of not more than 850 bases.
The full-length in vitro transcript directed the in vitro synthesis of a protein
which is precipitated by IgGanti ABP. Rabbit antibodies raised against a fusion
protein detect the ABP as a double band on Western blots. Only the smaller of
the two ABP bands is labeled by two different KDEL-specific IgG preparations.