Reference "Using amplified fragment length polymorphisms and simple sequence length polymorphisms to identify cultivars of brown and white milled rice. [Erratum: July/Aug 2000, v. 40 (4), p. 1188.]"

Reference ID

7020

Title

Using amplified fragment length polymorphisms and simple sequence length polymorphisms to identify cultivars of brown and white milled rice. [Erratum: July/Aug 2000, v. 40 (4), p. 1188.]

Source

Crop Science, 1999, vol. 39, pp. 1715-1721

Authors (4)

Bligh-H-F-J	Blackhall-N-W
Edwards-K-J	McClung-A-M

Abstract

Increase in world rice consumption combined with the recent World Trade
Organization General Agreement on Trade and Tariffs agreement on import tariffs
have resulted in a need for a method to distinguish between white milled rice
(Oryza sativa L.) cultivars sold at a premium from other milled cultivars. We
have used two DNA-based methods, amplified fragment length polymorphisms (AFLPs)
and simple sequence length polymorphisms (SSLPs), to distinguish six genetically
related long-grain rice cultivars, and a premium Basmati cultivar. Of the two
methods, AFLPs proved difficult to reproduce due to the quality of DNA that
could be extracted from the samples, while SSLPs showed sufficient variation to
distinguish all seven cultivars with only a small number of markers. Our study
demonstrates that, using white milled grains, SSLPs are a more robust and
efficient method than AFLPs for the identification of rice cultivars. We also
report the development of six new SSLP primer sets.