Reference "Adh1 is transcriptionally active but its translational product is reduced in a rad mutant of rice (Oryza sativa L.), which is vulnerable to submergence stress"

Reference ID

7009

Title

Adh1 is transcriptionally active but its translational product is reduced in a rad mutant of rice (Oryza sativa L.), which is vulnerable to submergence stress

Source

Theoretical and applied genetics, 1998, vol. 97, pp. 1197-1203

Authors (4)

Matsumura-H	Takano-T
Takeda-G	Uchimiya-H

Abstract

Enzyme activities and transcripts of alcohol dehydrogenase (ADH) were analyzed
in a single recessive rice (Oryza sativa L.) mutant with reduced ADH activity
(rad). It has been previously demonstrated that all ADH isozymes are below the
level of detection in aerobically grown rad seedlings (Matsumura et al. 1995).
The present study showed that ADH activity was anaerobically inducible in rad
seedlings and predominantly localized in the roots, mainly in the root-cap
region. The lowest band (ADH2 homodimer) of the ADH isozymes was observed in
submerged rad seedlings. Although two isozymes (the ADH1 homodimer and
heterodimer), abundant in the wild-type, were lost in this mutant, Adh1 mRNA was
produced normally in aerated or submerged rad plants. Protein analysis showed
that the amount of ADH protein was reduced in rad seedlings. Most likely, Adh1
mRNA was not translated or else its product was unstable in rad plants. The
viability of submerged rad seedlings was evaluated by the growth and greening of
the shoots. Our results showed that rad seedlings were more vulnerable to a long-
term submergence stress than wild-type seedlings.