Reference ID | 6931 | ||||||
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Title | PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.) | ||||||
Source | Theoretical and applied genetics, 2000, vol. 101, pp. 364-371 | ||||||
Authors (5) |
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Abstract | The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co- dominant PCR-based marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles from non-fragrance-producing alleles at the fgr locus. |
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