Reference "Mapping of four major rice blast resistance genes from ‘Lemont’ and ‘Teqing’"

Reference ID

6885

Title

Mapping of four major rice blast resistance genes from ‘Lemont’ and ‘Teqing’

Source

Theoretical and applied genetics, 2000, vol. 101, pp. 1215-1225

Authors (6)

Tabien-R-E	Li-Z
Paterson-A-H	Marchetti-M-A
Stansel-J-W	Pinson-S-R-M

Abstract

Abstract A framework linkage map was developed using 284 F10 recombinant inbred
lines (RILs) from a ‘Lemont’ ×‘Teqing’ rice cultivar cross. Evaluation of a
subset of 245 of these RILs with five races of the rice blast pathogen permitted
RFLP mapping of three major resistance genes from Teqing and one major gene from
Lemont. All mapped genes were found to confer resistance to at least two blast
races, but none conferred resistance to all five races evaluated. RFLP mapping
showed that the three resistance genes from Teqing, designated Pi-tq5, Pi-tq1
and Pi-tq6, were present on chromosomes 2, 6 and 12, respectively. The
resistance gene from Lemont, Pi-lm2, was located on chromosome 11. Pi-tq1 is
considered a new gene, based on its reaction to these five races and its unique
map location, while the other three genes may be allelic with previously
reported genes. Lines with different gene combinations were evaluated for
disease reaction in field plots. Some gene combinations showed both direct
effects and non-linear interaction. The fact that some of the lines without any
of the four tagged genes exhibited useful levels of resistance in the field
plots suggests the presence of additional genes or QTLs affecting the blast
reaction segregating in this population.